Curation Information

Iron and quorum sensing coordinately regulate the expression of vulnibactin biosynthesis in Vibrio vulnificus.;Wen Y, Kim IH, Son JS, Lee BH, Kim KS;The Journal of biological chemistry 2012 Aug 3; 287(32):26727-39 [22696215]
SmcR [Q7ME71, view regulon]
Reported TF sp.
Vibrio vulnificus MO6-24/O
Reported site sp.
Vibrio vulnificus MO6-24/O
Created by
Dinara Sagitova
Curation notes

Experimental Process

vvsA-luxAB reporter fusion showed that vvsA expression was highly increased in the smcR mutant compared to the wild type strain. RT-PCR showed that introduction of a wild-type smcR plasmid complemented the defect in vvsA transcription in the smcR mutant. Primer extension analysis determined the vvsA transcription start site. EMSA confirmed that SmcR binds specifically to the vvsA promoter. DNase I footprinting identified that SmcR protected a 22 bp region containing an inverted repeat sequence. 7 bases of the SmcR binding site were mutated by site-directed mutagenesis. LuxAB reporter assays with promoters containing wild-type and mutated SmcR binding sites showed that wild-type SmcR binding site was an essential cis-acting element for SmcR regulation of vvsA transcription.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVMO6_04199 VVMO6_04198 VVMO6_04197 VVMO6_04200
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified