Primer extension analyses performed on mRNA from wild type and ccpA mutant strains showed that bglH transcription was repressed about 10 fold in the wild type strain compared to the ccpA mutant. In vivo footprinting assay confirmed binding of CcpA to the bglH promoter at the putative CcpA binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|