Curation Information

Direct transcriptional control of the plasminogen activator gene of Yersinia pestis by the cyclic AMP receptor protein.;Kim TJ, Chauhan S, Motin VL, Goh EB, Igo MM, Young GM;Journal of bacteriology 2007 Dec; 189(24):8890-900 [17933899]
CRP [Q7CFV3, view regulon]
Reported TF sp.
Yersinia pestis GYS475
Reported site sp.
Yersinia pestis GYS475
Created by
Grace Chandler
Curation notes
The reported binding site did not match the genome. Possibly a bad proxy strain.

Experimental Process

A pla-lacZ fuison determined CRP activates pla expression. Visual inspection of the pla upstream region revealed a sequence similar to the CRP binding motif in E. coli. EMSA confirmed binding to the pla upstream region. DNase I footprinting localized this binding site. Site-directed mutagenesis in conjunction with pla-lacZ fusion assays demonstrated the specificity of CRP binding.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type