Curation Information

Publication

BswR controls bacterial motility and biofilm formation in Pseudomonas aeruginosa through modulation of the small RNA rsmZ.;Wang C, Ye F, Kumar V, Gao YG, Zhang LH;Nucleic acids research 2014 Apr; 42(7):4563-76 [24497189]

TF

BswR [Q9I063, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Dinara Sagitova

Curation notes

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Experimental Process

Analysis of the PrsmZ-directed b-gal. activity showed that deletion of bswR decreased the rsmZ expression, and overexpression of bswR increased the rsmZ expression. Consistent with these results, real-time RT-PCR assay also showed that the transcript level of rsmZ was decreased by 60% in the bswR-deleted mutant and increased by 3-fold in the bswR-overexpression strain. EMSA confirmed that BswR binds to the promoter region of rsmZ. DNase I footprinting assay identified the BswR binding region.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.