Curation Information

Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.;Mota LJ, Sarmento LM, de Sá-Nogueira I;Journal of bacteriology 2001 Jul; 183(14):4190-201 [11418559]
AraR [D4G1L9, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Joe Sparenberg
Curation notes

Experimental Process

lacZ assay results suggest that AraR represses the araABDLMNPQ-abfA operon. Site directed mutagenesis showed that mutations to the binding site increased expression of the araABDLMNPQ-abfA operon. in vitro transcription confirmed that AraR repressed the araABDLMNPQ-abfA operon. DNase I footprinting determined the binding sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type