Curation Information

Publication

Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.;Mota LJ, Sarmento LM, de Sá-Nogueira I;Journal of bacteriology 2001 Jul; 183(14):4190-201 [11418559]

TF

AraR [D4G1L9, view regulon]

Reported TF sp.

Bacillus subtilis subsp. subtilis str. 168

Reported site sp.

Bacillus subtilis subsp. subtilis str. 168

Created by

Joe Sparenberg

Curation notes

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Experimental Process

lacZ assay results suggest that AraR represses the araABDLMNPQ-abfA operon. Site directed mutagenesis showed that mutations to the binding site increased expression of the araABDLMNPQ-abfA operon. in vitro transcription confirmed that AraR repressed the araABDLMNPQ-abfA operon. DNase I footprinting determined the binding sequence.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.