Curation Information

Publication: Transcriptional regulatory cascade for elastase production in Vibrio vulnificus: LuxO activates luxT expression and LuxT represses smcR expression.;Roh JB, Lee MA, Lee HJ, Kim SM, Cho Y, Kim YJ, Seok YJ, Park SJ, Lee KH;The Journal of biological chemistry 2006 Nov 17; 281(46):34775-84 [16971386]
TF: LuxO [A0A0H0Y234, view regulon]
Reported TF sp.: Vibrio vulnificus MO6-24/O
Reported site sp.: Vibrio vulnificus MO6-24/O
Created by: Erill Lab
Curation notes: -

Experimental Process

Identified LuxO site in the promoter of LuxT by visual inspection with reference to known consensus. Used site directed mutagenesis coupled with luciferase assays with a luxO- mutant to demonstrate that the site is functional and activates LuxT transcription.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

GTTGCACCTAGCAAG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
GTTGCACCTAGCAAG	VVMO6_03374,		Experimental technique details Luciferase reporter assay (ECO:0005648) Luciferase reporter assay - ECO:0005648 A type of reporter assay . The idea is identical to a betagalactosidase assay, but here fluorescence of luciferase gene product is used as the reporter. - Experimental technique details Site directed mutagenesis (ECO:0005667) Site directed mutagenesis - ECO:0005667 Target-specific mutation, as opposed to non-specific mutation. In the context of TF-binding sites, site-directed mutagenesis is typically used to establish/confirm the specific sequence and location of a site, often in tandem with EMSA. Different positions of a putative binding site are mutated to non-consensus (or random) bases and binding to the mutated site is evaluated through EMSA or other means. Often implemented only in conserved motif positions or serially through all positions of a site. - Experimental technique details Visual sequence inspection (nan) Visual sequence inspection - nan Visual inspection of promoter region to identify putative binding sites based on previous knowledge -	activator	not specified