Curation Information

Cooperative regulation of the Vibrio vulnificus nan gene cluster by NanR protein, cAMP receptor protein, and N-acetylmannosamine 6-phosphate.;Kim BS, Hwang J, Kim MH, Choi SH;The Journal of biological chemistry 2011 Nov 25; 286(47):40889-99 [21956110]
CRP [Q7M7I9, view regulon]
Reported TF sp.
Vibrio vulnificus ATCC 29307
Reported site sp.
Vibrio vulnificus ATCC 29307
Created by
Dinara Sagitova
Curation notes

Experimental Process

The transcription start sites of nanT and nanEK nagA were determined by primer extension analyses. Comparison of the expression levels of the wild-type and crp mutant strains by qRT-PCR revealed that CRP activated nanT expression. EMSA showed that CRP bound specifically to the nanT-nanE intergenic region. DNase I footprinting revealed that CRP protected a region containing a CRP consensus sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVA1203 VVA1204 VVA1205 VVA1206 VVA1202 VVA1201 VVA1200 VVA1199 VVA1198
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified