The transcription start sites of nanT and nanEK nagA were determined by primer extension analyses. Comparison of the expression levels of the wild-type and crp mutant strains by qRT-PCR revealed that CRP activated nanT expression. EMSA showed that CRP bound specifically to the nanT-nanE intergenic region. DNase I footprinting revealed that CRP protected a region containing a CRP consensus sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TGTGATCGCGAACAGA||VVA1203, VVA1204, VVA1205, VVA1206,||