Curation Information

Publication
Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52.;Dong TG, Mekalanos JJ;Nucleic acids research 2012 Sep; 40(16):7766-75 [22723378]
TF
RpoN [Q9KP49, view regulon]
Reported TF sp.
Vibrio cholerae O37 strain V52 [185332]
Reported site sp.
Vibrio cholerae O37 strain V52 [185332]
Created by
Erill Lab
Curation notes
ChIP-PCR validated on Ch-2 Vibrio cholerae O1 biovar El Tor str. N16961 chosen as reference by authors (gene names)

Experimental Process

ChIP-seq, validated by qPCR in several genes RNA-seq, validated also by qPCR in a few genes

Transcription Factor Binding Sites


TGGCATTGAGTTTGC
TGGCATTCTTTATGC
TGGCATCAAATATGC
CGGCATGCTTTCTGC
TGGCACGTAATCTGC
TGGCATTGAGTTTGC
TGGCATTCTTTATGC
TGGCATCAAATATGC
CGGCATGCTTTCTGC
TGGCACGTAATCTGC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGGCATTGAGTTTGC VCA0123,
... ... VCA0123 VCA0122 VCA0121 VCA0120 VCA0119 VCA0118 VCA0117 VCA0116 VCA0115 VCA0114 VCA0113 VCA0112 VCA0111 VCA0110 VCA0109 VCA0108 VCA0107 VCA0124
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details RNA-Seq (ECO:0005664) - activator monomer
TGGCATTCTTTATGC
... ... VCA0554 VCA0553 VCA0555
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details RNA-Seq (ECO:0005664) - activator monomer
TGGCATCAAATATGC
... ... VCA0868 VCA0867 VCA0869 pbpG
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details RNA-Seq (ECO:0005664) - activator monomer
CGGCATGCTTTCTGC
... ... VCA0925 VCA0924 VCA0926
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details RNA-Seq (ECO:0005664) - activator monomer
TGGCACGTAATCTGC
... ... VCA0959 VCA0958 VCA0960
Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details ChIP-Seq (ECO:0006009) - Experimental technique details RNA-Seq (ECO:0005664) - activator monomer