Curation Information

Publication: CcpA mediates the catabolite repression of tst in Staphylococcus aureus.;Seidl K, Bischoff M, Berger-Bächi B;Infection and immunity 2008 Nov; 76(11):5093-9 [18710862]
TF: CcpA [Q2G295, view regulon]
Reported TF sp.: Staphylococcus aureus subsp. aureus N315
Reported site sp.: Staphylococcus aureus subsp. aureus N315
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

A putative CcpA binding site was identified in the tst promoter region by screening S. aureus N315 with the CcpA consensus sequence. Multiple sequence alignment confirmed that this sequence was conserved in tst promoters of the strains N315 and RF122. Luciferase reporter assays identified that glucose-dependent repression of tst transcription required CcpA.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

TATGTAAACGTTTACACA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Gene diagram	Experimental techniques	TF function	TF type
TATGTAAACGTTTACACA	tst		Experimental technique details Consensus search (ECO:0005624) Consensus search - ECO:0005624 This is a weak form of in-silico search, in which the consensus sequence for the motif is compared to genomic positions and the number of mismatches (between candidate site and consensus) is used as a measure of site-quality. - Experimental technique details Luciferase reporter assay (ECO:0005648) Luciferase reporter assay - ECO:0005648 A type of reporter assay . The idea is identical to a betagalactosidase assay, but here fluorescence of luciferase gene product is used as the reporter. - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) Multiple sequence alignment (MSA) - ECO:0005556 MSA can be used to align promoter regions and highlight regions of conservation that may be indicative of transcription factor binding sites. -	repressor	not specified