Curation Information

The alternative oxidase (AOX) gene in Vibrio fischeri is controlled by NsrR and upregulated in response to nitric oxide.;Dunn AK, Karr EA, Wang Y, Batton AR, Ruby EG, Stabb EV;Molecular microbiology 2010 Jul 1; 77(1):44-55 [20487270]
NsrR [Q5E2D6, view regulon]
Reported TF sp.
Vibrio fischeri ES114
Reported site sp.
Vibrio fischeri ES114
Created by
Dinara Sagitova
Curation notes

Experimental Process

aox transcription start site was determined by primer extension analysis. NsrR-mediated repression of aox was confirmed by comparing aox-lacZ expression levels in the wild type V. fischeri ES114 and the nsrR mutant strains. The results of this assay showed that Paox-lacZ activity was approximately 290-fold higher in the nsrR mutant strain. A putative NsrR binding site in the aox promoter was predicted by in silico analysis in a previous study. Site-directed mutagenesis of the NsrR binding site abolished NsrR-mediated repression as shown by aox-lacZ reported assays.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... aox VF_0577
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified