Curation Information

Publication

The LuxR-type regulator VpsT negatively controls the transcription of rpoS, encoding the general stress response regulator, in Vibrio cholerae biofilms.;Wang H, Ayala JC, Benitez JA, Silva AJ;Journal of bacteriology 2014 Mar; 196(5):1020-30 [24363348]

TF

VpsT [A0A0H3Q9Z3, view regulon]

Reported TF sp.

Vibrio cholerae C7258

Reported site sp.

Vibrio cholerae C7258

Created by

Dinara Sagitova

Curation notes

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Experimental Process

EMSA confirmed that VpsT binds to the rpoS promoter in the presence of c-di-GMP. ChIP assay showed that VpsT-FLAG can bind the rpoS promoter in vivo. An rpoS transcription start site was determined by primer extension analysis. DNase I footprinting identified the VpsT-protected region in the rpoS promoter. An rpoS-luxCDABE promoter fusion assays showed that expression of rpoS in the vpsT mutant was elevated compared to its wild type strain.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.