Curation Information

Regulation of the catechol 1,2-dioxygenase- and phenol monooxygenase-encoding pheBA operon in Pseudomonas putida PaW85.;Kasak L, Hôrak R, Nurk A, Talvik K, Kivisaar M;Journal of bacteriology 1993 Dec; 175(24):8038-42 [8253692]
CatR [Q88GK5, view regulon]
Reported TF sp.
Pseudomonas putida PaW85
Reported site sp.
Pseudomonas putida plasmid pEST1226
Created by
Matthew Coveyou
Curation notes

Experimental Process

Alignment of the pheBA and catBC promoter regions revealed the presence of two highly homologous CatR binding sites in these promoters. The functionality of the CatR binding site in pheBA promoter was confirmed by promoter deletion analysis. Comparison of pheBA expression levels in wild type and catR mutant strains suggested that CatR acted as a transcriptional activator.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type