Curation Information

Publication
The effect of specific rhlAlas-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR.;González-Valdez A, Servín-González L, Juárez K, Hernández EA, Soberón-Chávez G;FEMS microbiology letters 2014 Jun 16; 1(1):1 [24935161]
TF
LasR [P54292, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Site-directed mutagenesis combined with promoter-lacZ fusion assays confirmed RhlR-mediated activation in P.aeruginosa PAO1.

Transcription Factor Binding Sites


TCCTGTGAAATCTGGCAGTT
TCCTGTGAAATCTGGCAGTT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCCTGTGAAATCTGGCAGTT rhlA,
... ... rhlA rhlB rhlR
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified