Curation Information

Publication
Functional characterization of the NfxB repressor of the mexCD-oprJ multidrug efflux operon of Pseudomonas aeruginosa.;Purssell A, Poole K;Microbiology (Reading, England) 2013 Oct; 159(Pt 10):2058-73 [23924707]
TF
NfxB [P32265, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

EMSA with fragments of nfxB-mexC intergenic region showed that two inverted repeats present in this region are required for NfxB binding.

Transcription Factor Binding Sites


TCAAAAGATCATTTGA
TCGAAAAAGTACCATTCGA
TCAAAAGATCATTTGA
TCGAAAAAGTACCATTCGA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type