Curation Information

Publication

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism.;McFall SM, Klem TJ, Fujita N, Ishihama A, Chakrabarty AM;Molecular microbiology 1997 Jun; 24(5):965-76 [9220004]

TF

CatR [Q88GK5, view regulon]

Reported TF sp.

Pseudomonas putida PRS3026

Reported site sp.

Pseudomonas putida plasmid pAC27

Created by

Matthew Coveyou

Curation notes

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Experimental Process

In-vitro transcription assay indicated clcABD activation by CatR even in the absence of its inducer (CCM), though activation is increased in the presence of CCM. Comparative DNase I protection assays between CatR and ClcR revealed the region protected by ClcR with its inducer (2-CM) is identical to that protected by CatR without CCM.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.