Curation Information

Pseudomonas aeruginosa PA14 cupD transcription is activated by the RcsB response regulator, but repressed by its putative cognate sensor RcsC.;Nicastro GG, Boechat AL, Abe CM, Kaihami GH, Baldini RL;FEMS microbiology letters 2009 Nov; 301(1):115-23 [19832907]
RcsB [A0A0H2ZHG6, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PA14
Reported site sp.
Pseudomonas aeruginosa PA14
Created by
Grace Chandler
Curation notes

Experimental Process

Visual inspection of the cupD upstream region identified a putative RcsB binding site. RACE PCR determined the transcriptional start site location of cupD. qRT-PCR demonstrated RcsB-mediated activation of cupD transcription. These results were confirmed with cupD'-lacZ reporter assays. Multiple sequence alignment of RcsB-binding regions and putative binding sites further characterized the RcsB binding motif.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TAAGAAACGTCCTAAA cupD1, cupD2, cupD3, cupD4, cupD5
... ... cupD1 cupD2 cupD3 cupD4 cupD5
Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details RACE PCR (ECO:0005661) - Experimental technique details Visual sequence inspection (nan) - activator not specified