Curation Information

Publication

The transcriptional repressor TtgV recognizes a complex operator as a tetramer and induces convex DNA bending.;Guazzaroni ME, Krell T, Gutiérrez del Arroyo P, Vélez M, Jiménez M, Rivas G, Ramos JL;Journal of molecular biology 2007 Jun 15; 369(4):927-39 [17482209]

TF

TtgV [V9UYV3, view regulon]

Reported TF sp.

Pseudomonas putida DOT-T1E

Reported site sp.

Pseudomonas putida DOT-T1E

Created by

Dinara Sagitova

Curation notes

The genome used is the same as the one reported in the publication. However, not even inexact matches are found. Strain Pseudomonas putida DOT-T1E.

Experimental Process

TtgV binding to a 42-bp region in the ttgV-ttgG intergenic region had been shown previously by DNase I footprinting and DMS footprinting assays. Isothermal titration calorimetry experiments revealed that a 34 bp region containing two inverted repeats corresponds to a functional operator recognized by TtgV. Removal of the left half-site of IR1 or alteration of the right half-site of IR2 prevented binding of TtgV to the target operator. Alteration of the left half-site of IR2 or removal of the right half-site of IR1 resulted in low albeit measurable binding of TtgV to its operator.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.