Curation Information

In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.;Rojas A, Segura A, Guazzaroni ME, TerĂ¡n W, Hurtado A, Gallegos MT, Ramos JL;Journal of bacteriology 2003 Aug; 185(16):4755-63 [12896994]
TtgV [V9UYV3, view regulon]
Reported TF sp.
Pseudomonas putida DOT-T1E
Reported site sp.
Pseudomonas putida DOT-T1E
Created by
Dinara Sagitova
Curation notes

Experimental Process

ttgG-lacZ and ttgV-lacZ reporter assays in the wild-type DOT-T1E and in the ttgV mutant strains identified that TtgV represses the expression of both ttgVW and ttgGHI operons. EMSA showed that TtgV bound to a 210-bp DNA fragment containing the sequence of the ttgV-ttgG intergenic region. Footprinting assays defined the region TtgV binding region within the ttgV-ttgG intergenic region.

Transcription Factor Binding Sites

Quantitative data format: Kd

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type