Curation Information

Publication
Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene.;Shiba T, Ishiguro K, Takemoto N, Koibuchi H, Sugimoto K;Journal of bacteriology 1995 Oct; 177(20):5872-7 [7592337]
TF
NfxB [P32265, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

nfxB transcription start site was determined by primer extension. EMSA confirmed that NfxB specifically binds to its own promoter region. DNase I footprinting assay identified that the region from -20 to +83 of nfxB was protected from DNase I digestion by NfxB. Negative autoregulation of nfxB was shown by performing nfxB-lacZ fusion assays.

Transcription Factor Binding Sites


ACCGTTGATTGAAAACGGATCGAATG
GTACTTTTTCGAGCTAATTGAGTCAA
TATTGTCTCAAATGATCTTTGA
CAGCTAATTCCTTTGGACGCGAGGCCAG
ACCGTTGATTGAAAACGGATCGAATG
GTACTTTTTCGAGCTAATTGAGTCAA
TATTGTCTCAAATGATCTTTTG
CAGCTAATTCCTTTGGACGCGAGGCCAG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type