DMS footprinting protected a 36-bp sequence in the ttgABC-ttgR intergenic region. EMSA showed that TtgR binds to a 40-bp sequence that includes the 36-bp sequence protected by DMS footprinting. Site directed mutagenesis and EMSA identified the minimum length of the sequence that binds TtgR. Isothermal titration calorimetry confirmed these results.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|