S1 nuclease protection analysis using an algR knockout mutant identified lower cyanide levels in the mutant, demonstrating the role of AlgR as an activator of the hcnAB operon. EMSA confirmed AlgR binding to the hcnA promoter region which contains a previously identified AlgR binding site. Quantitative phenotypic analysis of HCN production showed AlgR activates cyanide production in the mucoid isolates and represses HCN production in non-mucoid isolates.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|