Curation Information

The transcriptional regulator AlgR controls cyanide production in Pseudomonas aeruginosa.;Carterson AJ, Morici LA, Jackson DW, Frisk A, Lizewski SE, Jupiter R, Simpson K, Kunz DA, Davis SH, Schurr JR, Hassett DJ, Schurr MJ;Journal of bacteriology 2004 Oct; 186(20):6837-44 [15466037]
AlgR [P26275, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

S1 nuclease protection analysis using an algR knockout mutant identified lower cyanide levels in the mutant, demonstrating the role of AlgR as an activator of the hcnAB operon. EMSA confirmed AlgR binding to the hcnA promoter region which contains a previously identified AlgR binding site. Quantitative phenotypic analysis of HCN production showed AlgR activates cyanide production in the mucoid isolates and represses HCN production in non-mucoid isolates.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... hcnA hcnB hcnC
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details S1 nuclease protection (ECO:0005666) - dual other