Curation Information

Publication

Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription.;Delic-Attree I, Toussaint B, Garin J, Vignais PM;Molecular microbiology 1997 Jun; 24(6):1275-84 [9218775]

TF

CysB [G3XCU6, view regulon]

Reported TF sp.

Pseudomonas aeruginosa CHA

Reported site sp.

Pseudomonas aeruginosa CHA

Created by

Grace Chandler

Curation notes

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Experimental Process

EMSA using DNA fragments of different sizes was used to confirm binding and localize the CysB binding site on the algD promoter. An algD-xylE fusion in wild type and cysB mutant strains demonstrated that CysB acts as an activator of algD expression.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.