Curation Information

Autoregulation of the Pseudomonas aeruginosa protein PtxS occurs through a specific operator site within the ptxS upstream region.;Swanson BL, Hamood AN;Journal of bacteriology 2000 Aug; 182(15):4366-71 [10894751]
PtxS [G3XD97, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

A ptxS-lacZ fusion identified PtxS as an auto-repressor, with ptxS expression levels four- to fivefold higher in the ptxS isogenic mutant. EMSA confirmed that PtxS binds specifically to a 52 bp fragment in the ptxS upstream region. DNase I footprinting further determined the location of a PtxS binding fragment. Site-directed mutagenesis combined with EMSA verified this fragment as a putative binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type