Curation Information

Publication

Autoregulation of the Pseudomonas aeruginosa protein PtxS occurs through a specific operator site within the ptxS upstream region.;Swanson BL, Hamood AN;Journal of bacteriology 2000 Aug; 182(15):4366-71 [10894751]

TF

PtxS [G3XD97, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Grace Chandler

Curation notes

-

Experimental Process

A ptxS-lacZ fusion identified PtxS as an auto-repressor, with ptxS expression levels four- to fivefold higher in the ptxS isogenic mutant. EMSA confirmed that PtxS binds specifically to a 52 bp fragment in the ptxS upstream region. DNase I footprinting further determined the location of a PtxS binding fragment. Site-directed mutagenesis combined with EMSA verified this fragment as a putative binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.