Curation Information

Publication
Arginine-dependent gene regulation via the ArgR repressor is species specific in chlamydia.;Schaumburg CS, Tan M;Journal of bacteriology 2006 Feb; 188(3):919-27 [16428395]
TF
ArgR [Q9Z8Z1, view regulon]
Reported TF sp.
Chlamydophila pneumoniae CWL029
Reported site sp.
Chlamydophila pneumoniae CWL029
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

An ArgR binding site in glnPQ upstream region was predicted via a bioinformatics approach in a previous study. In this publication, EMSA confirmed that ArgR specifically bound to the glnPQ promoter in the presence of L-arginine. In vitro transcription assay showed that ArgR represses transcription of the glnPQ promoter.

Transcription Factor Binding Sites


ATTGCATAAATATGATTT
TATAAATAAATATGCATA
ATTGCATAAATATGATTT
TATAAATAAATATGCATA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
ATTGCATAAATATGATTT glnP, glnQ,
... ... glnP glnQ argR gcp_2 oppA_1
Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - repressor not specified
TATAAATAAATATGCATA glnP, glnQ
... ... glnP glnQ
Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - repressor not specified