Curation Information

Activity of purified QscR, a Pseudomonas aeruginosa orphan quorum-sensing transcription factor.;Lee JH, Lequette Y, Greenberg EP;Molecular microbiology 2006 Jan; 59(2):602-9 [16390453]
PhzR [G3XD77, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

EMSA demonstrated QscR binding to the promoter region of PA1897. DNase I footprinting revealed a 20-bp inverted repeat sequence protected by QscR in the presence of 3OC12. Beta-gal assays performed with and without 30C12 confirmed that QscR directly activates the expression of PA1897. Functionality of the QscR binding site was validated by site-directed mutagenesis in conjunction with EMSAs.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... PA1897 PA1896 PA1895 PA1894 PA1893 PA1892 PA1891 PA1890 qscR
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator dimer