A consensus search identified the putative binding site. Site expression was induced in PhoB variable strains via phosphate starvation, and GUS assay indicated expression dependence on PhoB. A Western blot of PhoB variable cell extracts confirmed a loss of expression in the absence of PhoB. EMSA identified strong binding to the putative site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|