Curation Information

The alternative sigma factor RpoN regulates the quorum sensing gene rhlI in Pseudomonas aeruginosa.;Thompson LS, Webb JS, Rice SA, Kjelleberg S;FEMS microbiology letters 2003 Mar 28; 220(2):187-95 [12670680]
RpoN [P49988, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PA01UNSW
Reported site sp.
Pseudomonas aeruginosa PA01UNSW
Created by
Grace Chandler
Curation notes

Experimental Process

A rpoN knockout mutant strain of P. aruginosa PA01UNSW was created to test the effect of RpoN on the expression of rhlI. A lacZ fusion assay determined that RpoN positively regulates rhlI transcription, demonstrating its role as an activator of rhIl. Alignment of promoter of genes known to be regulated by RpoN identified an RpoN consensus sequence (YTGGCACGrNNNTTGCW).

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... rhlI
Experimental technique details Beta-gal reporter assay - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - activator not specified