Curation Information

Choline catabolism, σ⁵⁴ factor and NtrC are required for the full expression of the Pseudomonas aeruginosa phosphorylcholine phosphatase gene.;Massimelli MJ, Sánchez DG, Buchieri MV, Olvera L, Beassoni PR, Schweizer HP, Morett E, Lisa AT;Microbiological research 2011 Jul 20; 166(5):380-90 [20869215]
RpoN [P49988, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

LacZ reporter assays in wild-type and rpoN mutant strains showed that β-galactosidase activity was highly reduced (≅75%) in the mutant. Site-directed mutagenesis in the putative −12 element reduced pchP expression by 95%. The pchP transcriptional start site was determined by 5' RACE experiment. The presence of TSS 14 nucleotides downstream of the putative σ54 −12 element, provided further evidence that this promoter was transcribed by this sigma factor. Additionally, a sequence resembling an IHF binding site was identified in the pchP promoter region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pchP
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified