Curation Information

Publication

FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site.;Jyot J, Dasgupta N, Ramphal R;Journal of bacteriology 2002 Oct; 184(19):5251-60 [12218010]

TF

FleQ [G3XCV0, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAK

Reported site sp.

Pseudomonas aeruginosa PAK

Created by

Dinara Sagitova

Curation notes

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Experimental Process

LacZ reporter assays in wild-type, fleQ and rpoN mutant strains confirmed FleQ and RpoN dependent regulation of the target promoters. EMSAs showed FleQ specifically binds to sequences within its target promoters. DNase I footprinting was used to identify FleQ binding sites. Site-directed mutagenesis of the FleQ binding site in the flhA promoter confirmed direct regulation by FleQ.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.