Curation Information

Publication
The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element.;den Hengst CD, van Hijum SA, Geurts JM, Nauta A, Kok J, Kuipers OP;The Journal of biological chemistry 2005 Oct 7; 280(40):34332-42 [16040604]
TF
CodY [A2RHP2, view regulon]
Reported TF sp.
Lactococcus lactis subsp. cremoris MG1363
Reported site sp.
Lactococcus lactis subsp. cremoris MG1363
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

To identify the genes that constitute the regulon of L. lactis CodY, the transcriptional profile of L. lactis MG1363 was compared with that of L. lactis MG1363ΔcodY. β-Galactosidase assays were used to confirm the DNA microarray results. EMSAs were performed to confirm that CodY binds its target promoters.

Transcription Factor Binding Sites


AATTGTCTGACAATT
ATTCGTCAGTAAATT
TTTTTTCAAAAAAAT
AATTGTCTGACAATT
ATTCGTCAGTAAATT
TTTTTTCAAAAAAAT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AATTGTCTGACAATT ctrA,
... ... ctrA llmg_0119
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - repressor not specified
ATTCGTCAGTAAATT ctrA,
... ... ctrA llmg_0117 llmg_0119
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - repressor not specified
TTTTTTCAAAAAAAT ctrA,
... ... ctrA llmg_0119
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - repressor not specified