Curation Information

XCC2731, a GGDEF domain protein in Xanthomonas campestris, is involved in bacterial attachment and is positively regulated by Clp.;Hsiao YM, Liu YF, Fang MC, Song WL;Microbiological research 2011 Oct 20; 166(7):548-65 [21237626]
Clp [Q4UZF6, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str.17
Reported site sp.
Xanthomonas campestris pv. campestris str.17
Created by
Dinara Sagitova
Curation notes

Experimental Process

5′ RACE method was used to identify the XCC2731 transcription start site. A predicted Clp-binding site TGAGCN6TCACA, with 7/10 matching the consensus sequence, was located at −87/−102 relative to the XCC2731 TIS. XCC2731–lacZ transcriptional fusion constructs were used to map the upstream region required for expression of the XCC2731 gene. EMSAs demonstrated that Clp binds its target promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... XCC2731 XCC2730
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - activator not specified