Curation Information

Publication
Systematic mutagenesis of all predicted gntR genes in Xanthomonas campestris pv. campestris reveals a GntR family transcriptional regulator controlling hypersensitive response and virulence.;An SQ, Lu GT, Su HZ, Li RF, He YQ, Jiang BL, Tang DJ, Tang JL;Molecular plant-microbe interactions : MPMI 2011 Sep; 24(9):1027-39 [21615202]
TF
HpaR1 [Q8PAI3, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str. 8004
Reported site sp.
Xanthomonas campestris pv. campestris str. 8004
Created by
Dinara Sagitova
Curation notes
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Experimental Process

HpaR1 binding site was identified by scanning promoter region of the hpaR1. Direct autoregulation of hpaR1 was confirmed by performing EMSA. In vitro transcription assay showed that HpaR1 can repress its own tran- scription level in vitro. hpaR1-GUS reporter assays showed that at HpaR1 can enhance its own expression in vivo when the bacterial cells are inside the host plant.

Transcription Factor Binding Sites


GTTGAATACAAAACCAGTCATCCAAC
GTTGAATACAAAACCAGTCATCCAAC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GTTGAATACAAAACCAGTCATCCAAC XC_2736,
... ... XC_2736 XC_2735 XC_2737 XC_2738 XC_2739 XC_2740 XC_2741
Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details Visual sequence inspection (nan) - not specified not specified