Curation Information

Clp and RpfF up-regulate transcription of pelA1 gene encoding the major pectate lyase in Xanthomonas campestris pv. campestris.;Hsiao YM, Fang MC, Sun PF, Tseng YH;Journal of agricultural and food chemistry 2009 Jul 22; 57(14):6207-15 [19601664]
Clp [Q4UZF6, view regulon]
Reported TF sp.
Xanthomonas campestris pv. campestris str.17
Reported site sp.
Xanthomonas campestris pv. campestris str.17
Created by
Dinara Sagitova
Curation notes

Experimental Process

Using the 5′ RACE method, the pelA1 transcription initiation site was mapped at nucleotide G, 103 nt upstream of the pelA1 start codon. Transcriptional fusion assays showed that Clp positively regulates pelA1 transcription. Gel retardation assays showed that Clp exerts a positive control over expression of pelA1 by direct binding to the upstream Clp-binding site

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pel
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Visual sequence inspection (nan) - activator not specified