Curation Information

Publication
Compartmentalized glucose metabolism in Pseudomonas putida is controlled by the PtxS repressor.;Daddaoua A, Krell T, Alfonso C, Morel B, Ramos JL;Journal of bacteriology 2010 Sep; 192(17):4357-66 [20581202]
TF
PtxS [Q88HH7, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Promoter-lacZ fusions carried out in the wild-type strain and in the ptxS-deficient strain confirmed that PtxS represses gene expression in the wild-type strain. EMSAs showed that PtxS binds to its target promoters. Also, by using EMSAs it was shown that 2-ketogluconate freed PtxS from its target sequence. After the transcription start sites were identfied, footprinting analysis showed that PtxS protects a single region within each of the promoters. In each of the protected regions a perfect palindromic sequence was found.

Transcription Factor Binding Sites


TGAAACCGGTTTCA
TGAAACCGGTTTCA
TGAAACCGGTTTCA
TGAAACCGGTTTCA
TGAAACCGGTTTCA
TGAAACCGGTTTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGAAACCGGTTTCA PP_3379,
... ... PP_3379 kguK PP_3377 kguD
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TGAAACCGGTTTCA ptxS,
... ... ptxS PP_3379 kguK PP_3377 kguD
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer
TGAAACCGGTTTCA PP_3382,
... ... PP_3382 PP_3384 PP_3383 PP_3385 vgrG-2 PP_3387 PP_3388 PP_3389
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - repressor dimer