Curation Information

Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa.;Thaden JT, Lory S, Gardner TS;Journal of bacteriology 2010 May; 192(10):2557-68 [20233934]
LasR [P25084, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

Transcriptional-profiling experiments in both the rich LB medium and the more well-defined modified FAB medium showed that cueR expression is positively controlled by 3OC12-HSL. A promoter-lacZ assay confirmed that cueR expression is activated by the Las signal 3OC12-HSL and is not influenced by the Rhl signal C4-HSL. The results from the transcriptional reporter assay in E. coli further confirmed that cueR is activated primarily by LasR/3OC12-HSL. EMSAs confirmed that LasR binds the upstream region of the cueR gene. The putative LasR binding motif in the cueR promoter was validated by generating a series of promoter-lacZ constructs with different mutations in the Las box.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... cueR pmrB
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified