Transcriptional-profiling experiments in both the rich LB medium and the more well-defined modified FAB medium showed that cueR expression is positively controlled by 3OC12-HSL. A promoter-lacZ assay confirmed that cueR expression is activated by the Las signal 3OC12-HSL and is not influenced by the Rhl signal C4-HSL. The results from the transcriptional reporter assay in E. coli further confirmed that cueR is activated primarily by LasR/3OC12-HSL. EMSAs confirmed that LasR binds the upstream region of the cueR gene. The putative LasR binding motif in the cueR promoter was validated by generating a series of promoter-lacZ constructs with different mutations in the Las box.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|