Curation Information

Publication
The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis.;Kovacikova G, Lin W, Skorupski K;Journal of bacteriology 2010 Aug; 192(16):4181-91 [20562308]
TF
AphB [Q9KT56, view regulon]
Reported TF sp.
Vibrio cholerae O1 biovar El Tor str. N16961
Reported site sp.
Vibrio cholerae O1 biovar El Tor str. N16961
Created by
Erill Lab
Curation notes
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Experimental Process

Array to detect likely regulated genes. EMSA to verify binding, beta-gal to confirm induction

Transcription Factor Binding Sites


TGTAACTAACTGTTTCA
AACAACCTAAGTTTGCA
AGCAAAATAAACTTACT
TGTAACTAACTGTTTCA
AACAACCTAAGTTTGCA
AGCAAAATAAACTTACT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTAACTAACTGTTTCA VC0770,
... ... VC0770 VC0769
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator dimer
AACAACCTAAGTTTGCA VC0278,
... ... VC0278 zntR
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator dimer
AGCAAAATAAACTTACT nhaB,
... ... nhaB VC1900
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details EMSA (ECO:0001807) - activator dimer