Curation Information

Publication: PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in Staphylococcus aureus.;Horsburgh MJ, Clements MO, Crossley H, Ingham E, Foster SJ;Infection and immunity 2001 Jun; 69(6):3744-54 [11349039]
TF: PerR [Q2G282, view regulon]
Reported TF sp.: Staphylococcus aureus subsp. aureus NCTC 8325
Reported site sp.: Staphylococcus aureus subsp. aureus NCTC 8325
Created by: Dinara Sagitova
Curation notes: -

Experimental Process

Putative PerR binding sites were identified in the S. aureus based on homology with the putative site upstream of katA. To determine whether these candidate PerR binding sites were located in the promoter region of the genes, transcriptional start point was determined. In each case, the PerR binding site was located in the promoter region close to the −35 and −10 elements. LacZ reporter fusions were used to confirm PerR-regulated expression of the identified genes.

Transcription Factor Binding Sites

Sites reported in the paper
Sites matched in the genome

AATTATAATTATTATAAAT
GATTAGAATTATTATAATT
GATTAGAATTATTATAATA
AATAATAATTATTATATAA
TTTTTTAATTATTAGTAGG
AATTATAATTATTATTATT
GCATATAATTATTATTATT
TCATAAAATTATTATAATG

AATTATAATTATTATAAAT
GATTAGAATTATTATAATT
GATTAGAATTATTATAATA
AATAATAATTATTATATAA
GATTTTAATTATTAGTAGG
AATTATAATTATTATTATT
GCATATAATTATTATTATT
TCATAAAATTATTATAATG

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence	Regulated genes	Experimental techniques	TF function	TF type
AATTATAATTATTATAAAT	SAOUHSC_01327,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
GATTAGAATTATTATAATT	SAOUHSC_00365, SAOUHSC_00364,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
GATTAGAATTATTATAATA	SAOUHSC_02381,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
AATAATAATTATTATATAA	SAOUHSC_01997,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
GATTTTAATTATTAGTAGG	SAOUHSC_01592,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
AATTATAATTATTATTATT	SAOUHSC_02108,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
GCATATAATTATTATTATT	SAOUHSC_00785,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified
TCATAAAATTATTATAATG	SAOUHSC_01999,	Experimental technique details Beta-gal reporter assay Beta-gal reporter assay Reporter assay using the beta-galactosidase (lacZ) gene. The lacZ gene is typically fused to the promoter of interest. Differential regulation of the promoter mediated by the TF is assessed by induction of the system and evaluation of lacZ expression. Bacteria expressing lacZ appear blue when grown on a X-gal medium. The assay is often performed using a plasmid borne construction on a lacZ(def) strain. -	repressor	not specified