Curation Information

Ribonucleotide reductases of Salmonella typhimurium: transcriptional regulation and differential role in pathogenesis.;Panosa A, Roca I, Gibert I;PloS one 2010 Jun 25; 5(6):e11328 [20593029]
Fur [Q7CQY3, view regulon]
Reported TF sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Reported site sp.
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Created by
Dinara Sagitova
Curation notes

Experimental Process

Fur-mediated regulation was shown by performing complementation assays. Site-directed mutagenesis of the putative Fur box in conjunction with nrdHIEF-lacZ reporter fusion confirmed Fur-mediated regulation of nrdHIEF. Specific binding of Fur to its target promoter was confirmed by performing EMSAs with a wild-type promoter and a promoter containing an altered Fur box.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type