Fur-mediated regulation was shown by performing complementation assays. Site-directed mutagenesis of the putative Fur box in conjunction with nrdHIEF-lacZ reporter fusion confirmed Fur-mediated regulation of nrdHIEF. Specific binding of Fur to its target promoter was confirmed by performing EMSAs with a wild-type promoter and a promoter containing an altered Fur box.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|