Curation Information

Fur controls iron homeostasis and oxidative stress defense in the oligotrophic alpha-proteobacterium Caulobacter crescentus.;da Silva Neto JF, Braz VS, Italiani VC, Marques MV;Nucleic acids research 2009 Aug; 37(14):4812-25 [19520766]
Fur [A0A0H3C2M1, view regulon]
Reported TF sp.
Caulobacter crescentus NA1000
Reported site sp.
Caulobacter crescentus NA1000
Created by
Dinara Sagitova
Curation notes

Experimental Process

Putative Fur-binding sites in the genome of C. crescentus were detected by using a position weight matrix approach. EMSA validated that Fur binds to its target promoter region. LacZ reporter assays validated Fur-mediated expression regulation. Site-directed mutagenesis of Fur-binding sites confirmed specific binding.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... CC_1956 CC_1955 CC_1954 CC_1953 CC_1952 CC_1951 CC_1950 CC_1949 CC_1948 CC_1947 CC_1946 CC_1945 CC_1944 CC_1943 CC_1942 CC_1941 CC_1940 CC_1939 CC_1938 CC_1937 CC_1936 CC_1935 CC_1934 CC_1933 CC_1932 CC_t26 CC_t27 CC_1957 CC_1958
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified