Curation Information

The quorum sensing regulator HapR downregulates the expression of the virulence gene transcription factor AphA in Vibrio cholerae by antagonizing Lrp- and VpsR-mediated activation.;Lin W, Kovacikova G, Skorupski K;Molecular microbiology 2007 May; 64(4):953-67 [17501920]
Lrp [Q9KQU4, view regulon]
Reported TF sp.
Vibrio cholerae C6706
Reported site sp.
Vibrio cholerae C6706
Created by
Elliot White
Curation notes

Experimental Process

Authors used B-gal assay to determine that Lrp activates aphA. Binding was confirmed with an EMSA. The proposed site is based off of the E. Coli consensus sequence

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VC0395_A2223 gpsA cysE VC0395_A2224 ppc
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - activator not specified