Authors used B-gal assay to determine that Lrp activates aphA. Binding was confirmed with an EMSA. The proposed site is based off of the E. Coli consensus sequence
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|