The consensus sequence of the PerR-box in S. pyogenes and B. subtilis was used to screen S. suis genome for putative PerR boxes. RT-PCR was performed to validate PerR-dependent transcription regulation of the identified genes. EMSAs showed that PerR could bind to its target promoters.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|