Curation Information

Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor.;Schröder I, Darie S, Gunsalus RP;The Journal of biological chemistry 1993 Jan 15; 268(2):771-4 [8419352]
IHF [P0A6X7, view regulon]
Reported TF sp.
Escherichia coli MC4100
Reported site sp.
Escherichia coli MC4100
Created by
Dinara Sagitova
Curation notes

Experimental Process

narG-lacZ reporter fusion was done to validate that IHF protein acts as an activator of narG expression. DNase I footprinting was performed to locate IHF binding site on the narG promoter. EMSA results showed that IHF specifically binds its target promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TAAAACGTCTTAA narG, narI, narJ, narH,
... ... narG narI narJ narH narK
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - activator other