Curation Information

Fur functions as an activator and as a repressor of putative virulence genes in Neisseria meningitidis.;Delany I, Rappuoli R, Scarlato V;Molecular microbiology 2004 May; 52(4):1081-90 [15130126]
Fur [P0A0S8, view regulon]
Reported TF sp.
Neisseria meningitidis MC58
Reported site sp.
Neisseria meningitidis MC58
Created by
Dinara Sagitova
Curation notes

Experimental Process

Putative Fur binding sites were identified by screening N. meningitidis MC58 genome with Fur-box sequence of E. coli. DNase I footprinting was used to demonstrate binding of Fur to the predicted consensus binding sites. S1 nuclease protection assay showed that Fur activated the expression of norB promoter. norB-lacZ reporter fusion showed that fur activates its target promoter directly.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... norB pan1 NMB1624
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details PSSM site search (ECO:0005659) - Experimental technique details S1 nuclease protection (ECO:0005666) - activator not specified