Curation Information

Publication
Reconstruction of the evolutionary history of the LexA-binding sequence.;Mazón G, Erill I, Campoy S, Cortés P, Forano E, Barbé J;Microbiology (Reading, England) 2004 Nov; 150(Pt 11):3783-95 [15528664]
TF
LexA [P0DN68, view regulon]
Reported TF sp.
Fibrobacter succinogenes subsp. succinogenes S85
Reported site sp.
Fibrobacter succinogenes subsp. succinogenes S85
Created by
Erill Lab
Curation notes
-

Experimental Process

PSSM search based on detailed mutagenesis of lexA promoter box to detect candidate sites. LexA-binding verified by EMSA.

Transcription Factor Binding Sites


AGCTCAAAGGCGCA
TGCCTCCTCGAGCA
TATTCAAATGTTCA
TGCACAAAAGTTCA
AGCTCAAAGGCGCA
TGCCTCCTCGAGCA
TATTCAAATGTTCA
TGCACAAAAGTTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AGCTCAAAGGCGCA
... ... Fisuc_0766 Fisuc_0761 Fisuc_0762 Fisuc_0763 Fisuc_0764 Fisuc_0765
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TGCCTCCTCGAGCA
... ... Fisuc_2967 Fisuc_2966 Fisuc_2965 Fisuc_2964 Fisuc_2963 Fisuc_2962 Fisuc_2950 Fisuc_2951 Fisuc_2952 Fisuc_2953 Fisuc_2954 Fisuc_2955 Fisuc_2956 Fisuc_2957 Fisuc_2958 Fisuc_2959 Fisuc_2960 Fisuc_2961
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TATTCAAATGTTCA
... ... Fisuc_0598 Fisuc_0597 Fisuc_0592 Fisuc_0593 Fisuc_0594 Fisuc_0595 Fisuc_0596
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TGCACAAAAGTTCA
... ... Fisuc_2376 Fisuc_2375 Fisuc_2370 Fisuc_2371 Fisuc_2372 Fisuc_2373 Fisuc_2374
Experimental technique details EMSA (ECO:0001807) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer