Curation Information

Publication
Expression of Vibrio vulnificus insulin-degrading enzyme is regulated by the cAMP-CRP complex.;Ryu Y, Kim YJ, Kim YR, Seok YJ;Microbiology (Reading, England) 2012 May; 158(Pt 5):1294-303 [22361942]
TF
CRP [Q7M7I9, view regulon]
Reported TF sp.
Vibrio vulnificus MO6-24/O
Reported site sp.
Vibrio vulnificus MO6-24/O
Created by
Erill Lab
Curation notes
-

Experimental Process

The location of the CRP that binds between two promoter revealed by DNase I footprinting assay and the in vitro transcription assays showed that CRP activates ideV PS transcription in the presence of cAMP. Electrophoretic mobility shift assays and site directed mutagenesis(EMSAs) used to identify the binding site of CRP in the ideV promoter.

The paper reports that TF forms complex with other proteins for binding with reported sites.

Expression of Vibrio vulnificus insulin-degrading enzyme is regulated by the cAMP–CRP complex.CRP activates ideV PS transcription in the presence of cAMP whose concentration is regulated by EIIAGlc

Transcription Factor Binding Sites


AGCGTTATCAATCTCA
AGCGTTATCAATCTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AGCGTTATCAATCTCA VVMO6_00860,
... ... VVMO6_00860 VVMO6_00861 VVMO6_00859 VVMO6_00858 VVMO6_00862 VVMO6_00863 VVMO6_00864
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator dimer