Curation Information

Publication

Mechanism for iron-regulated transcription of the Escherichia coli cir gene: metal-dependent binding of fur protein to the promoters.;Griggs DW, Konisky J;Journal of bacteriology 1989 Feb; 171(2):1048-54 [2644221]

TF

Fur [P0A9A9, view regulon]

Reported TF sp.

Escherichia coli str. K-12 substr. MG1655

Reported site sp.

Escherichia coli str. K-12 substr. MG1655

Created by

Urooq Aqeel

Curation notes

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Experimental Process

First a B-gal assay was performed to confirm fur expression. Gel retardation assays were used to demonstrate binding of purified Fur protein to the iron control region. Finally a footprint analysis done using DNAse I, found the TF binding site.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.