Curation Information

Publication
The SOS response of Listeria monocytogenes is involved in stress resistance and mutagenesis.;van der Veen S, van Schalkwijk S, Molenaar D, de Vos WM, Abee T, Wells-Bennik MH;Microbiology (Reading, England) 2010 Feb; 156(Pt 2):374-84 [19892760]
TF
LexA [Q8Y7H7, view regulon]
Reported TF sp.
Listeria monocytogenes EGD-e
Reported site sp.
Listeria monocytogenes EGD-e
Created by
Erill Lab
Curation notes
sites validated by RT-PCR
External databases
NCBI GEO (GSE12634) http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12634.

Experimental Process

DNA array for w-t and recA mutant after exposure to mit-C to identify target genes. Validation with RT-PCR for some genes (recA, yneA, lexA, bilEA and dinB). Motif discovery on genes upregulated only in w-t background (i.e. RecA-dependent)

Transcription Factor Binding Sites


AAAAAGAATGTATGTTCGCTT
AAAGCGAACATACATTCTTTT
TGTACGAACGGTTGTTCTATA
AATACGAATAAATGTTCGCTT
ATATAGAACATACATTCGATT
AATAAGAACGCTTGTTCGTTT
AAAAAGAATGTATGTTCGCTT
AAAGCGAACATACATTCTTTT
TGTACGAACGGTTGTTCTATA
AATACGAATAAATGTTCGCTT
ATATAGAACATACATTCGATT
AATAAGAACGCTTGTTCGTTT

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AAAAAGAATGTATGTTCGCTT lmo1302, lmo1303,
... ... lmo1302 lmo1303 lmo1300 lmo1301
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
AAAGCGAACATACATTCTTTT lmo1302, lmo1303,
... ... lmo1302 lmo1303 lmo1301 lmo1300
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
TGTACGAACGGTTGTTCTATA lmo1302, lmo1303,
... ... lmo1302 lmo1303 lmo1301 lmo1300
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
AATACGAATAAATGTTCGCTT recA,
... ... recA cinA
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
ATATAGAACATACATTCGATT lmo1421, lmo1422,
... ... lmo1421 lmo1422 murB lmo1419 lmo1423
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer
AATAAGAACGCTTGTTCGTTT lmo1975,
... ... lmo1975 lmo1976 lmo1977
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor dimer