Curation Information

Publication
The zinc-responsive regulon of Neisseria meningitidis comprises 17 genes under control of a Zur element.;Pawlik MC, Hubert K, Joseph B, Claus H, Schoen C, Vogel U;Journal of bacteriology 2012 Dec; 194(23):6594-603 [23043002]
TF
Zur [Q9JZ70, view regulon]
Reported TF sp.
Neisseria meningitidis MC58
Reported site sp.
Neisseria meningitidis MC58
Created by
Dinara Sagitova
Curation notes
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Experimental Process

Zur-regulated genes were identified by DNA microarray analysis. Zur-mediated expression regulation was confirmed by qRT-PCR experiments. Promoter regions of the identified genes were scanned for the presence of a Zur box by alignments using ClustalW. Also, upstream regions were searched for conserved motifs with the GIBBS Motif Sampler.

Transcription Factor Binding Sites


TGTTATGTTATAACA
TGTTTTACCATAACA
TATAATGGATTAACA
CGTTATACAGTATCA
CGATATGTCAAACCA
AGGTCTATAATGCCA
TGTAATCCATTTGCA
ATACACTTTTTAACA
TGTTACGCTATATCA
TGTTATGTTATAACA
TGTTTTACCATAACA
TATAATGGATTAACA
CGTTATACAGTATCA
CGATATGTCAAACCA
AGGTCTATAATGCCA
TGTAATCCATTTGCA
ATACACTTTTTAACA
TGTTACGCTATATCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TGTTATGTTATAACA rpmE2,
... ... rpmE2 rpmJ metF
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
TGTTTTACCATAACA NMB0586,
... ... NMB0586 NMB0587 NMB0588
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
TATAATGGATTAACA NMB0317,
... ... NMB0317 farA farB
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
CGTTATACAGTATCA NMB0588,
... ... NMB0588 NMB0587 rplS trmD rimM rpsP NMB0593
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
CGATATGTCAAACCA NMB0525,
... ... NMB0525 rbn NMB0523 NMB0526 NMB0527
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
AGGTCTATAATGCCA NMB0817,
... ... NMB0817 NMB0816 NMB0818
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
TGTAATCCATTTGCA NMB0820,
... ... NMB0820 NMB0819
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
ATACACTTTTTAACA NMB0819,
... ... NMB0819 NMB0818 NMB0817 NMB0816
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified
TGTTACGCTATATCA NMB1497,
... ... NMB1497 NMB1496 lysC
Experimental technique details DNA-array expression analysis (ECO:0005525) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified