Curation Information

Publication
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.;Ochsner UA, Vasil ML;Proceedings of the National Academy of Sciences of the United States of America 1996 Apr 30; 93(9):4409-14 [8633080]
TF
Fur [Q03456, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Kathryn Cronise
Curation notes
-

Experimental Process

A SELEX-like approach was used to identify putative sites. EMSA and DNase footprinting were used to extract sites that bind Fur.

Transcription Factor Binding Sites


TCTTCTGATAATTATTATC
CTGAATGATAATAATTATC
ACGAACGTGAATCATTCTC
TCTTCTGATAATTATTATC
CTGAATGATAATAATTATC
ACGAACGTGAATCATTCTC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCTTCTGATAATTATTATC
... ... PA5532 tonB1
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Genomic SELEX (ECO:0005634) - repressor not specified
CTGAATGATAATAATTATC
... ... PA5532 tonB1
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Genomic SELEX (ECO:0005634) - repressor not specified
ACGAACGTGAATCATTCTC
... ... pfeS PA2684 PA2685 pfeR
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Genomic SELEX (ECO:0005634) - repressor not specified