Curation Information

Positive regulation of flhDC expression by OmpR in Yersinia pseudotuberculosis.;Hu Y, Wang Y, Ding L, Lu P, Atkinson S, Chen S;Microbiology (Reading, England) 2009 Nov; 155(Pt 11):3622-31 [19643764]
OmpR [Q7CFX0, view regulon]
Reported TF sp.
Yersinia pseudotuberculosis YPIII
Reported site sp.
Yersinia pseudotuberculosis YPIII
Created by
Elliot White
Curation notes

Experimental Process

Primer extension was used to determine the gene start-site and show that there was promoter activity between +50 and -150. Two candidate sites were identified using a cited consensus sequence, and were confirmed via B-gal assay to upregulate flhDC. Binding was then confirmed with site directed mutagenesis and a series of EMSA's. The EMSAs showed significantly more binding at site 2 than site 1.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... YPK_1745 YPK_1746 YPK_1744
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator monomer